Procedure :
1. Put 1 ml of the glucose reagent in each of two small test tubes.
2. Withdraw 10? of serum by micropipette and put them in one of the two tubes and sign it as test tube.
3. Withdraw 10? of glucose standard and put them in the other tube and sign it as standard tube.
4. Leave the two tubes in water bath for 10 minutes or in room temperature for 20 minutes.
5. Use the wave length 500 nm for measuring.
6. Put the standard and record the reading then put the tested serum and record the reading .
Calculations:
[The reading of test / The reading of standard ] * conc. of glucose standard .
(usually 100mg/dl)
Normal values :
FBS (70 -115 mg/dl) fast blood sugar (fasting 8 hrs) .
RBS (up to 125 mg/dl) random blood sugar
PPBS (80 – 140 mg/dl) postprandial blood sugar (2 hrs after meal)