Hansel eosinophils stain

Reagents

Hansel stain ( this reagent is obtained from a scientific vendor)

Procedure

Prepare a smear or a cytospin of the sediment. Nature or dilute 50:50.

1. Let the smear dry at room temperature
2. Fix 5 sec. with methanol 95%.
3. Add to the slide 25 drops of Hansel stain, 45 sec.
4. Add to the slide 25 drops of H2O, 30 sec
5. Rinse with water, clean the bottom of the slide with methanol.
6. Let dry.
7. Mount the slide with Eukitt (facultative)
8. Read with the 100x objective.