In case of hypertension cholesterol may in high levels.
In this test patient must fasting at least 12 – 14 hrs.

Procedure:
The reagent may ready to use or must be prepared according to the kits of the reagents.

Prepare 1 ml of reagent in each of two test tubes.
Put 10 µ of standard in one of the two tubes an mark it as standard tube.
Put 10 µ of serum in the other tube and mark it as test tube.
Leave for 5 minutes in water bath or for 10 minutes in room temp.
Measure at wave length 505 nm.

Calculation:
(Reading of test / reading of standard) x conc. Of standard (usually 200 mg/dl)

Normal:
Less than 200 mg/dl.
Border line 220 – 239 mg/dl.
The high value starts from 240.

Note:
If the reading cannot appear in the colorimeter you should dilute the serum by 0.9% saline in ratio 1: 1
then the reading multiply x 2 .