Preparation of the dye:
- Powder blue enamel dissolved Bmekdra 1 gm in 100 ml solution composed of 80 ml saline (9 g of salt in a liter) and 20 mM sodium citrate solution 3.1%. And then run the solution after preparation.
- Retinal pigmentation of the cell in blue enamel to be distinguished from the rest of the red blood cells.
Reticulocyte Hb may be better test of infants' iron.(Clinical Rounds): An article from: Pediatric News
Way:
- Put in a test tube and Zerman 3 drops of dye blue enamel.
- Add the blood of any weakness 6 drops of blood taken at'd clot in normal situations such as in cases Alanemiae add 12 points from the blood and mix well.
- Then put the tube in the incubation temperature of 37 degrees for 30 minutes.
- We get a clean slide by and take a tube of absorbent Iastair acetobutylicum and put a drop of blood on the slide and are keen to several films, as in the normal test CNN NBC.
- Wait until the dry films, and then prepared for examination through the lens of oil.
Algorithm:
Choose the place where there is no match of red blood cells on each so you can see the reticulocyte which is a red blood cells containing the remains of chromatin threadsAnd reticulocyte count should be in ten fields at least see the number of red blood cells in this number, preferably counted in the 1000 football red blood.
Method:
Reticulocyte Reticulocytes \u003d
(Total number of cells in the film for ten fields / total number of red blood cells for ten fields) * 100 \u003d ....%
Ratios of natural: 0.2 - 2.0%
 | Blood reticulocytes |
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