in case of rheumatic fever and streptococcal infection.
Procedure :
*Reagent must have the room temp. before use then mix gently.
1.Put 10 µ of serum in a black slide then put on it 10 µ of the reagent .
2.Mix and rotate the slide by hands in a circle direction for 2 min .
-If precipitation occurs the result + ve. and give titre 200 IU / ml .
-If no ppt occurs the result is -ve .
-In +ve case we must repeat by the following steps:
1.Serum diluted by saline by the ratio 1 : 1 .
2.Take 10 µ from mix and put on the black slide then put 10 µ reagent mix and rotate for 2 min.
-If -ve so the titre 200 IU /ml .
-If +ve the titre 400 IU /ml . and repeat by the dilution of last mix by the ratio 1 : 1 or dilute the serum directly by The ratio 1 serum : 2 saline .and so on till the result give -ve .
The Titre in this test 200 - 400 - 600 - 800 IU/ml and no higher than 800 IU/ml .
IMPORTANT COMMENT:
-->False +ve result may occur in early infection and children between 6 months to 2 years .
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