Platelets Count Test
Platelets Count Test:
Platelet count test
There are no perfect way in this test due to the ease of platelet aggregation and the small size so that should be used clean tools and solutions used to be sterilized and filteredAlways placed in the refrigerator when not in use and be sure they are free from impurities.
There are two ways to test:
1. Direct Direct
2. Indirect Indirect
1. The direct method
Mode of formalin citrate Formalin Citrate:
Attending formalin solution citrate as follows:
32 gm sodium citrate in 1 liter dist water + 10 ml formalin
32 grams of sodium citrate per liter of water + 10 ml formalin
Way:
1 - is placed in a test tube and Zerman 2 cm of a solution of formalin jackets.
2 - draw blood from the sucking up to 2 mark.
3 - placed in the solution and mix well.
4 - point taken by dropper and placed on a slide count.
5 - is in the red boxes and count hits in 1000.
Mode of aluminum oxalate:
1 g of ammonium oxalate in 100 ml distilled water and preserves and will nominate a refrigerator before use.
Way:
1 - take the blood clot'd EDTA.
2 - Blood taken Balmasp specific to red blood cells up to the mark and continue on to the mark 100 Baoxalat ammonium and mix the contents of the absorbers.
3 - put on a clean slide the Cover and keep it part of the contents of the absorbers.
4 - put the slide in a Petri-Dish contains a wet cotton or paper nomination wet and covered dish and leave for 20 minutes at a temperature of 15-25 degrees Celsius.
5 - put the slide under the microscope count is platelets Ktrivp used to count red blood collected boxes and hit results in 5000.
2. The method of indirect
Platelet count in a way the film painted with the same character of Reticulocytes with the difference in platelet count of the patient must follow the following:
- Back on the red blood Alheimustiomitr of the patient.
- Put a drop of oil Alsidar on the part of the film painted homogeneous (ie part flour).
- Prepare red blood cells found in the movie with back plates.
- We start to count up the number of red blood cells 1000 a ball and see the number of platelets in this number.
Method:
Platelet count \u003d (number of platelets in the film, dyed / number of red balls in the film painted) x number of red blood cells of the patient
Normal values: 150000-450000
http://laboratorymedicne.blogspot.com/
http://visualbasicall.blogspot.com/
http://medicalwindows.blogspot.com/
http://www.saad6.com
Platelet count test
There are no perfect way in this test due to the ease of platelet aggregation and the small size so that should be used clean tools and solutions used to be sterilized and filteredAlways placed in the refrigerator when not in use and be sure they are free from impurities.
There are two ways to test:
1. Direct Direct
2. Indirect Indirect
1. The direct method
Mode of formalin citrate Formalin Citrate:
Attending formalin solution citrate as follows:
32 gm sodium citrate in 1 liter dist water + 10 ml formalin
32 grams of sodium citrate per liter of water + 10 ml formalin
Way:
1 - is placed in a test tube and Zerman 2 cm of a solution of formalin jackets.
2 - draw blood from the sucking up to 2 mark.
3 - placed in the solution and mix well.
4 - point taken by dropper and placed on a slide count.
5 - is in the red boxes and count hits in 1000.
Mode of aluminum oxalate:
1 g of ammonium oxalate in 100 ml distilled water and preserves and will nominate a refrigerator before use.
Way:
1 - take the blood clot'd EDTA.
2 - Blood taken Balmasp specific to red blood cells up to the mark and continue on to the mark 100 Baoxalat ammonium and mix the contents of the absorbers.
3 - put on a clean slide the Cover and keep it part of the contents of the absorbers.
4 - put the slide in a Petri-Dish contains a wet cotton or paper nomination wet and covered dish and leave for 20 minutes at a temperature of 15-25 degrees Celsius.
5 - put the slide under the microscope count is platelets Ktrivp used to count red blood collected boxes and hit results in 5000.
2. The method of indirect
Platelet count in a way the film painted with the same character of Reticulocytes with the difference in platelet count of the patient must follow the following:
- Back on the red blood Alheimustiomitr of the patient.
- Put a drop of oil Alsidar on the part of the film painted homogeneous (ie part flour).
- Prepare red blood cells found in the movie with back plates.
- We start to count up the number of red blood cells 1000 a ball and see the number of platelets in this number.
Method:
Platelet count \u003d (number of platelets in the film, dyed / number of red balls in the film painted) x number of red blood cells of the patient
Normal values: 150000-450000
http://laboratorymedicne.blogspot.com/
http://visualbasicall.blogspot.com/
http://medicalwindows.blogspot.com/
http://www.saad6.com
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