Fragility Test

Solutions used and the tools:

- Salt solution concentration of 1% (1 gm in 100 ml distilled water).
- Rak solid wood by 15 test tube, 13 of them for testing and 2 of them to control.


Way:
- Put in the pipelines of different amounts of distilled water and salt solution be given different concentrations of between 20% to 80%



The following table shows the amounts added to test tubes and Control Unit Anpopty painted dark gray 14 and 15 and Almekdaer established in centimeters (cm):

Concentration ratio \u003d amount of poison the minds of salt solution / amount of poison the minds of all of the tube (ie, saline + distilled water)

- Then leave for an hour to two hours and then read and reading, we can identify the beginning and end of the cracker.

Normal values:


The beginning of cracking: 0.40% - 0.45%
The end of the cracker: 0.30% - 0.35%

The conditions:

Alhishaship useful test in identifying certain types of anemia and is tested in her example, so if they started breaking balls in the concentration higher than that of say 0.70% And the end of the cracker to 50% had anemia Svirosetozis this case because there are red balls full Alastdrap any can not hold its shape long explodes quickly.
But if it was the beginning of cracking at a concentration of less than that at 50% and was 20% at the end of this indicates that there are two types of anemia anemia either the Mediterranean or sickle cell anemia and thatBecause red blood Alastdrap be incomplete and take the form of sickle or crescent therefore take a long time to leak out to the salt concentration is cracker.

Notes:

- The test is conducted to determine the change in red blood cells and resistance to permeated if placed in salt solution is less concentrated than the Tabay and salt solution.
- Must take into account accuracy in the development of brine and distilled water because it affects the final result.
Hemolysis by ultrasound: A comparative study of the osmotic and ultrasonic fragility tests (Scandinavian journal of haematology)
- Must be considered to be equal to the amount of blood does not happen in the end, we find an error occurred by cracking tube and other tube did not occur by cracking.
- When reading must first make sure if the Control Unit Control distilled water burst of red blood cells and if all control brine will remain as they are all either if you findOtherwise he shall be deemed an error and re-test than the first.
- When you shake tubes must be taken not turned over between thumb and index finger so as not to move the concentration of the solution from the tube to another, and thereby increasing the concentration of the other tube and gives an error in the resultTherefore must shake Bmskha pipe from the top (of the crater) and shake hands on the belly to prevent the error occurs. 



http://laboratorymedicne.blogspot.com/
http://visualbasicall.blogspot.com/ 
http://medicalwindows.blogspot.com/
http://www.saad6.com