Toluidine blue staining procedure
Reagent
150 mg of Toluidine blue dissolve in PBS buffer.Hank's solution (Difco)
PBS buffer: phosphate buffered saline solution (Sigma)
Technique
- In a test tube, add 3 drops of sediment.
- Add 1 ml of PBS buffer or Hank's solution (Difco)
- add 1 or 2 drops of stain depending on cellularity
- Centrifuge
- Aspirate the added fluid volume and read