ASEPTIC TECHNIQUE
ASEPTIC TECHNIQUE: In handling bacteria specimens, do everything possible to assure that extraneous bacteria from you do not contaminate the specimen…
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The microscope
The microscopical examination of the urinary sediment is a difficult job. The examiner becomes rapidly uncomfortable even exhausted …
Acetate filter PAP stain for the urinary cytodiagnostic
Reagents Filter (Gelman GN6 13 mm) or (Millipore RAW 013 ) Filter holder sweeny 13 mm Hank's solution Hématoxiline acide free Orange G ( Orth…
Naphtyl AS-D Chloroacetate esterase
Principle The aim of this stain is to demonstrate the presence of granulocytes. Granulocyte lysozomes contain a rather specific hydrolase that …
Hansel eosinophils stain
Reagents Hansel stain ( this reagent is obtained from a scientific vendor) Procedure Prepare a smear or a cytospin of the sediment. Nature or …
Toluidine blue staining procedure
Reagent 150 mg of Toluidine blue dissolve in PBS buffer. Hank's solution (Difco) PBS buffer: phosphate buffered saline solution (Sigma) Techn…
Sediment conservation Staining methods
Specimen conservation Several solutions are possible for the conservation of interesting specimens. The simplest consists in adding an equal…